Mobilization of the non-conjugative plasmid RSF1010: A genetic analysis of its origin of transfer

Abstract

The oriT site of the broad host-range multicopy IncQ plasmid RSF1010 was cloned onto the 2.2 kb pBR322-derived vector pED825. By successive subcloning and construction of deletions, the oriT region was localised on an 80-88 bp segment of DNA. This segment was contained within the HaeII fragment of RSF1010 that is known to include the relaxation nick site… (More)
DOI: 10.1007/BF00326551

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