Mobilisation of Ca2+ stores and flagellar regulation in human sperm by S-nitrosylation: a role for NO synthesised in the female reproductive tract.

@article{MachadoOliveira2008MobilisationOC,
  title={Mobilisation of Ca2+ stores and flagellar regulation in human sperm by S-nitrosylation: a role for NO synthesised in the female reproductive tract.},
  author={Gisela S M Machado-Oliveira and Linda Lefi{\`e}vre and Christopher Ford and Mar{\'i}a Bel{\'e}n Herrero and Christopher Barratt and Thomas J. Connolly and Katherine Nash and Aduen Morales-Garcia and Jackson C. Kirkman-Brown and Steve J Publicover},
  journal={Development},
  year={2008},
  volume={135 22},
  pages={3677-86}
}
Generation of NO by nitric oxide synthase (NOS) is implicated in gamete interaction and fertilisation. Exposure of human spermatozoa to NO donors caused mobilisation of stored Ca(2+) by a mechanism that did not require activation of guanylate cyclase but was mimicked by S-nitroso-glutathione (GSNO; an S-nitrosylating agent). Application of dithiothreitol, to reduce protein -SNO groups, rapidly reversed the actions of NO and GSNO on [Ca(2+)](i). The effects of NO, GSNO and dithiothreitol on… CONTINUE READING

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