MnlI--The member of H-N-H subtype of Type IIS restriction endonucleases.

  title={MnlI--The member of H-N-H subtype of Type IIS restriction endonucleases.},
  author={Edita Kriukienė and Judita Lubiene and Arūnas Lagunavi{\vc}ius and Arvydas Lubys},
  journal={Biochimica et biophysica acta},
  volume={1751 2},
The Type IIS restriction endonuclease MnlI recognizes the non-palindromic nucleotide sequence 5'-CCTC(N)7/6 downward arrow and cleaves DNA strands as indicated by the arrow. The genes encoding MnlI restriction-modification system were cloned and sequenced. It comprises N6-methyladenine and C5-methylcytosine methyltransferases and the restriction endonuclease. Biochemical studies revealed that MnlI restriction endonuclease cleaves double- and single-stranded DNA, and that it prefers different… CONTINUE READING

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