Mn2+ suppressor mutations and biochemical communication between Ty1 reverse transcriptase and RNase H domains.

@article{Yarrington2007Mn2SM,
  title={Mn2+ suppressor mutations and biochemical communication between Ty1 reverse transcriptase and RNase H domains.},
  author={Robert M. Yarrington and Jichao Chen and Eric C Bolton and Jef D. Boeke},
  journal={Journal of virology},
  year={2007},
  volume={81 17},
  pages={9004-12}
}
Ty1 reverse transcriptase/RNase H (RT/RH) is exquisitely sensitive to manganese concentrations. Elevated intracellular free Mn(2+) inhibits Ty1 retrotransposition and in vitro Ty1 RT-polymerizing activity. Furthermore, Mn(2+) inhibition is not limited to the Ty1 RT, as this ion similarly inhibits the activities of both avian myeloblastosis virus and human immunodeficiency virus type 1 RTs. To further characterize Mn(2+) inhibition, we generated RT/RH suppressor mutants capable of increased Ty1… CONTINUE READING