Mitotic chromosome doubling of plant tissues in vitro

@article{Dhooghe2010MitoticCD,
  title={Mitotic chromosome doubling of plant tissues in vitro},
  author={Emmy Dhooghe and Katrijn Van Laere and Tom Eeckhaut and Leen Leus and Johan M. Van Huylenbroeck},
  journal={Plant Cell, Tissue and Organ Culture (PCTOC)},
  year={2010},
  volume={104},
  pages={359-373}
}
In vitro chromosome doubling can be induced by several antimitotic agents. The most commonly used are colchicine, oryzalin and trifluralin. The process of induced chromosome doubling in vitro consists of a typical succession of sub-processes, including an induction phase and a confirmation protocol to measure the rate of success. The induction step depends on a large number of variables: media, antimitotic agents, explant types, exposure times and concentrations. Flow cytometry is the pre… 

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