Mitotic chromosome doubling of plant tissues in vitro

  title={Mitotic chromosome doubling of plant tissues in vitro},
  author={Emmy Dhooghe and Katrijn Van Laere and Tom Eeckhaut and Leen Leus and Johan M. Van Huylenbroeck},
  journal={Plant Cell, Tissue and Organ Culture (PCTOC)},
In vitro chromosome doubling can be induced by several antimitotic agents. The most commonly used are colchicine, oryzalin and trifluralin. The process of induced chromosome doubling in vitro consists of a typical succession of sub-processes, including an induction phase and a confirmation protocol to measure the rate of success. The induction step depends on a large number of variables: media, antimitotic agents, explant types, exposure times and concentrations. Flow cytometry is the pre… 


The spontaneous diploidization rates in oilseed rape (Brassica napus L.) via in vitro androgenesis are too low for practical applications. In contrast, artificial doubling of chromosomes of the


The spontaneous diploidization rates in oilseed rape (Brassica napus L.) via in vitro androgenesis are too low for practical applications. In contrast, artificial doubling of chromosomes of the

Artificial polyploidy in the improvement of horticultural crops

Overall, polyploidy manipulation has long been used in improving the yield of many crop plants and can be considered as one of the most promising tools in plant breeding programs.

Chromosome doubling in Cattleya tigrina A

Chromosome doubling induction in orchids may benefit their production for resulting in flowers of higher commercial value, larger size and higher content of substances that intensify the color and

Improving in vitro induction of autopolyploidy in grapevine seedless cultivars

This is the first report of in vitro oryzalin induced polyploidization of grapevine and of the use of mathematical modeling to optimize chromosome doubling in plants.

Artificial polyploidy in medicinal plants: Advancement in the last two decades and impending prospects

The current review gives an overview of the different parameters of in vitro chromosome doubling, the history of the technique, and progress made over the last two decades.

Studies on Colchicine Induced Chromosome Doubling for Enhancement of Quality Traits in Ornamental Plants

Flow cytometry and chromosome counting, with an observation of morphological and physiological traits are routine procedures for the determination of ploidy level in plants.

In vitro Ploidy Manipulation for Crop Improvement

An overview of mitotic inhibitors and their application for in vitro ploidy manipulation for plant breeding and crop improvement is provided.

Effect of Polyploidy Induction on Natural Metabolite Production in Medicinal Plants

Polyploidy plays an important role in plant diversification and speciation. The ploidy level of plants is associated with morphological and biochemical characteristics, and its modification has been



In vitro chromosome doubling of Miscanthus sinensis

An efficient chromosome doubling method for Miscanthus sinensis to enable the production of triploids and so avoid seed dispersal to the environment was developed and resulted in a high degree of albinism.

In liquid medium colchicine treatment induces non chimerical doubled-diploids in a wide range of mono- and interspecific diploid banana clones

This study has clearly shown that induction of stable doubled-diploid plants can be obtained from a wide range of genetically different bananas, and open the way to the systematic use of doubled- diploids by banana breeding programs for the release of enhanced triploid varieties.

In vitro procedures for the induction of tetraploidy in a diploid rose

It is anticipated that a satisfactory yield of non-chimeral tetraploids could be regenerated from such tissues from plantlets cultured in vitro in liquid medium and treated with spindle inhibitors or tritiated thymidine.

Mitotic blocking, micronucleation, and chromosome doubling by oryzalin, amiprophos-methyl, and colchicine in potato

Flow cytometric determination of nuclear DNA content in interphase cells and the analysis of chromosome numbers in mitotic cells after removal of the chemicals and subculture showed that oryzalin is the most efficient chromosome doubling agent followed by APM and colchicine in that order.

Role of mitotic inhibitors and genotype on chromosome doubling of Rosa

Flow cytometry results at 18 and 24 weeks after herbicide treatment, indicated that the best time to test the treated plants was after 24  weeks, which suggest that chromosome doubling is genotype dependent and plants with lower ploidy level have a higher propensity for chromosome doubling.

MOLECULAR BIOLOGY AND PHYSIOLOGY Towards Improved Cell Cycle Synchronization and Chromosome Preparation Methods in Cotton

A procedure for cell cycle synchronization of root tips using hydroxyurea (HU), and the efficacy of several known chemicals for metaphase accumulation are reported, which allowed the production of high quality chromosome preparations and showed little ability to increase metaphase indices.

Physiology of polyploids.

  • M. Tal
  • Biology
    Basic life sciences
  • 1979
Future work on the physiology of autopolyploid plants should be directed towards: the investigation of more representative experimental systems that should include genetically homogeneous and heterogeneous species of both wild and cultivated plants; and diploid-polyploid comparisons on the level of isolated tissues and cells, in addition to the whole plant.

Oryzalin-induced Chromosome Doubling in Buddleja to Facilitate Interspecific Hybridization

A protocol for producing fertile tetraploid forms of the hybrid Buddleja madagascarensis Lam. x B. crispa Benth. would enable introgression of orange flower, pubescence, and nondehiscent fruit

Induction of Fertile Amphidiploids by Artificial Chromosome-doubling in Interspecific Hybrid between Dianthus caryophyllus L. and D. japonicus Thunb

The amphidiploids induced to restore the fertility by artificial chromosome-doubling exhibited a larger flower size and later flowering time than the original diploid hybrid, and the fertility of both pollen and seed was restored.