Mitosis enhances transgene expression of plasmid delivered by cationic liposomes.

@article{Tseng1999MitosisET,
  title={Mitosis enhances transgene expression of plasmid delivered by cationic liposomes.},
  author={Wen Chieh Tseng and Frederick R Haselton and Todd D Giorgio},
  journal={Biochimica et biophysica acta},
  year={1999},
  volume={1445 1},
  pages={53-64}
}
A critical requirement of gene therapy is expression of the delivered transgene. Transgene expression is facilitated by access to the transcription mechanism found primarily in the nucleus. Factors modulating the interactions between intracellular plasmid and nuclear access are not well understood. In this study, the effect of mitosis on transgene expression was examined by quantitative flow cytometry. Transfection of HeLa cells synchronized at late G1 phase or G2/M phase was performed using a… CONTINUE READING

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