Mitomycin C-induced apoptosis in cultured human Tenon's capsule fibroblasts.

@article{Kim1999MitomycinCA,
  title={Mitomycin C-induced apoptosis in cultured human Tenon's capsule fibroblasts.},
  author={Jin Woo Kim and Sang Kyum Kim and In Ho Song and In Taek Kim},
  journal={Korean journal of ophthalmology : KJO},
  year={1999},
  volume={13 1},
  pages={7-15}
}
To investigate the mitomycin C-induced apoptotic cell death of fibroblasts, the primarily cultured human Tenon's capsule fibroblasts were exposed to a clinically used dosage of 0.4 mg/ml of mitomycin C for 5 minutes. TUNEL (TdT-mediated dUTP-biotin nick end labeling) assay and electron microscopic studies were performed to determine the extent of mitomycin C-induced apoptosis. A flow cytometric study was performed to quantify the apoptotic cell population over time. The TUNEL stains were… CONTINUE READING