Mitogenic Signaling Mediated by Oxidants in Ras-Transformed Fibroblasts

@article{Irani1997MitogenicSM,
  title={Mitogenic Signaling Mediated by Oxidants in Ras-Transformed Fibroblasts},
  author={Kaikobad Irani and Yong Xia and Jay L. Zweier and Steven J. Sollott and Channing J. Der and Eric R. Fearon and Maitrayee Sundaresan and Toren Finkel and Pascal J. Goldschmidt-Clermont},
  journal={Science},
  year={1997},
  volume={275},
  pages={1649 - 1652}
}
NIH 3T3 fibroblasts stably transformed with a constitutively active isoform of p21Ras, H-RasV12 (v-H-Ras or EJ-Ras), produced large amounts of the reactive oxygen species superoxide (·O2−). ·O2− production was suppressed by the expression of dominant negative isoforms of Ras or Rac1, as well as by treatment with a farnesyltransferase inhibitor or with diphenylene iodonium, a flavoprotein inhibitor. The mitogenic activity of cells expressing H-RasV12 was inhibited by treatment with the chemical… Expand
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