Mitogenic Properties of Insulin-Like Growth Factors I and II, Insulin-Like Growth Factor Binding Protein-3 and Epidermal Growth Factor on Human Breast Epithelial Cells in Primary Culture

  title={Mitogenic Properties of Insulin-Like Growth Factors I and II, Insulin-Like Growth Factor Binding Protein-3 and Epidermal Growth Factor on Human Breast Epithelial Cells in Primary Culture},
  author={Karen S. Strange and Darcy A. Wilkinson and Joanne T. Emerman},
  journal={Breast Cancer Research and Treatment},
Insulin-like growth factor-I (IGF-I) and insulin-like growth factor-II (IGF-II) are growth factors implicated in mammary gland development and are believed to be involved in breast cancer. However, the interactions between components of the IGF system and breast epithelial cells, which give rise to breast cancer, are not well understood. We have investigated the mitogenic properties of IGF-I, IGF-II, IGF binding protein-3 (IGFBP-3) and epidermal growth factor (EGF) on human breast epithelial… 

Mitogenic Properties of Insulin-Like Growth Factors I and II, Insulin-like Growth Factor Binding Protein-3 and Epidermal Growth Factor on Human Breast Stromal Cells in Primary Culture

The mitogenic properties of IGF-I, IGF-II, insulin-like growth factor binding protein-3 (IGFBP-3) and epidermal growth factor (EGF) on human breast stromal cells in primary culture are investigated to aid understanding of the mechanisms behind the role of the IGF system in breast cancer.

Epidermal growth factor induces insulin receptor substrate-2 in breast cancer cells via c-Jun NH(2)-terminal kinase/activator protein-1 signaling to regulate cell migration.

EGF remarkably induced expression of major IGF signaling components, insulin receptor substrate (IRS)-1 and IRS-2, an effect that could be blocked by EGF receptor (EGFR) tyrosine kinase inhibitors, revealing a novel mechanism of cross-talk between the EGF and IGF signaling pathways.

Insulin-like growth factor-I receptor expression and localization in benign and malignant rat mammary tumors.

Investigation of the expression and localization of the insulin growth factor type 1 receptor in malignant and benign mammary tumors induced in rats by N-nitroso-N-methylurea and its correlation with histopathology and hormone dependence suggests a different expression and role of IGF-IR in benign and malignant tumors.

Insulin-Like Growth Factor-I, IGF-Binding Protein-3, and Mammographic Breast Density

It is confirmed that IGF-I and IGFBP-3 are associated with breast density among premenopausal women, and this provides additional support for the idea that these growth factors may affect breast cancer risk, at least in part, through their influence on breast tissue morphology as reflected on mammogram.

Genetic Polymorphisms Involved in Insulin-like Growth Factor (IGF) Pathway in Relation to Mammographic Breast Density and IGF Levels

Findings provide additional support for the idea that several components of the IGF pathway may affect breast cancer risk and that this effect on breast cancer development may be mediated, at least in part, through its influence on the morphogenesis of breast tissue.

Growth Factor Regulation of Cell Cycle Progression in Mammary Epithelial Cells

This review addresses what is currently known about how growth factors regulate the cell cycle in mammary/breast epithelial cells both individually and in coordination with other growth regulators.

The insulin-like growth factor family and breast cancer prognosis: A prospective cohort study among postmenopausal women in Denmark.

  • Loa KalledsøeL. Dragsted A. Olsen
  • Medicine, Biology
    Growth hormone & IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society
  • 2019

A quantitative RT-PCR study of the mRNA expression profile of the IGF axis during mammary gland development.

This study has demonstrated the importance and complexity of the IGF axis during mammary gland development and provides a valuable resource for future research in this area.

Reports: Insulin-Like Growth Factor-I, Soy Protein Intake, and Breast Cancer Risk

It is suggested that soy protein intake may negatively modulate the effect of IGF-I and may positively modulateThe effect of insulin-like growth factor-I levels on premenopausal breast cancer risk.



Insulin-like growth factor-II (IGF-II): a potential autocrine/paracrine growth factor for human breast cancer acting via the IGF-I receptor.

Insulin-like growth factor-II was slightly less potent than IGF-I in stimulating DNA synthesis in MCF-71 cells, an effect that paralleled its ability to compete for [125I]IGF-I binding in these cells.

Effects of insulin-like growth factors (IGFs) and IGF receptor antibodies on the proliferation of human breast cancer cells.

It is indicated that nanomolar concentration of insulin, IGF-I and IGF-II are potent mitogens in MCF-7 cells and can potentially stimulate cell proliferation through all three receptors.

Insulin-like growth factor binding protein-3 induces apoptosis in MCF7 breast cancer cells.

The results suggest that IGFBP-3 enhances apoptosis by reducing bioavailability of ligands for the IGF-I receptor and suggest that modulation of IGF BP-3 expression by ICI contributes to apoptosis induced by this compound, suggesting that IGFBPs are regulators of apoptosis.

Estradiol and Antiestrogens Regulate a Growth Inhibitory Insulin-like Growth Factor Binding Protein 3 Autocrine Loop in Human Breast Cancer Cells (*)

Evidence is provided for an estradiol and antiestrogen-regulated IGFBP-3 growth inhibitory autocrine pathway in MCF-7 cells.

Plasma insulin-like growth factor-1 (IGF-1) concentrations in human breast cancer.

Regulation of insulin-like growth factors by antiestrogen

Assumption that tumor regression from antiestrogens is mainly observed in ER-positive type based on IGF regulation alone is certainly an oversimplistic view amid the complexity of autocrine, paracrine, and endocrine functions.

The insulin-like growth factor family of ligands, receptors, and binding proteins

There is great potential for complex interactions between the family members that could ultimately regulate normal and neoplastic cell growth.

Insulin-like growth factors and their binding proteins: biological actions.

In recognition of its generalized pleiotypic actions, sulfation factor was renamed somatomedin (mediator of the effects of somatotropin) and was included in the emerging classification of broad spectrum growth factors along with platelet derived growth factor, fibroblast growth factors, and epidermal growth factor.

Effect of tamoxifen on serum insulinlike growth factor I levels in stage I breast cancer patients.

It is concluded that tamoxifen treatment reduces IGF-I levels and that this reduction may contribute to the therapeutic effect of the drug.