Mitogen-driven B cell proliferation and differentiation are not accompanied by hypermutation of immunoglobulin variable region genes.

Abstract

Hybridomas were constructed from splenic B cells after mitogen stimulation in vitro with lipopolysaccharide and dextran sulfate for 9 to 11 days. Extensive proliferation and differentiation (secretion of IgG isotypes) was evident in these cultures before fusion. Hybridomas that express a VH gene segment whose germ-line sequence is known were isolated, and… (More)

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Cite this paper

@article{Manser1987MitogendrivenBC, title={Mitogen-driven B cell proliferation and differentiation are not accompanied by hypermutation of immunoglobulin variable region genes.}, author={Timothy Manser}, journal={Journal of immunology}, year={1987}, volume={139 1}, pages={234-8} }