Mitochondrial remnant organelles of Giardia function in iron-sulphur protein maturation

@article{Tovar2003MitochondrialRO,
  title={Mitochondrial remnant organelles of Giardia function in iron-sulphur protein maturation},
  author={Jorge Tovar and Gloria Le{\'o}n-Avila and Lidya B. S{\'a}nchez and Robert Sutak and Jan Tachezy and Mark van der Giezen and Manuel Hern{\'a}ndez and Mikl{\'o}s M{\"u}ller and John Milton Lucocq},
  journal={Nature},
  year={2003},
  volume={426},
  pages={172-176}
}
Giardia intestinalis (syn. lamblia) is one of the most widespread intestinal protozoan pathogens worldwide, causing hundreds of thousands of cases of diarrhoea each year. Giardia is a member of the diplomonads, often described as an ancient protist group whose primitive nature is suggested by the lack of typical eukaryotic organelles (for example, mitochondria, peroxisomes), the presence of a poorly developed endomembrane system and by their early branching in a number of gene phylogenies. The… Expand
Localization and functionality of microsporidian iron–sulphur cluster assembly proteins
TLDR
The essential biosynthetic process of Fe–S protein assembly as a key function of microsporidian mitosomes is identified. Expand
Giardia mitosomes and trichomonad hydrogenosomes share a common mode of protein targeting.
TLDR
It is reported that proteins targeted into mitosomes of Giardia intestinalis have targeting signals necessary and sufficient to be recognized by the mitosomal protein import machinery, and a shared mode of protein targeting supports the hypothesis that mitosome, hydrogenosomes, and mitochondria represent different forms of the same fundamental organelle having evolved under distinct selection pressures. Expand
The Mitochondrion-Related Organelles of Blastocystis
TLDR
This chapter presents and examines the status quo regarding the biology and morphology of Blastocystis mitochondrion-related organelles (MROs), and discusses future avenues on exploring even further adaptations of these organlles. Expand
Inheritance of the reduced mitochondria of Giardia intestinalis is coupled to the flagellar maturation cycle
TLDR
It is proposed that the flagellar system controls segregation of mitochondrial organelles also in other members of this supergroup (Metamonada) of eukaryotes and perhaps reflects the original strategy of early eukARYotic cells to maintain this key organelle before mitochondrial fusion-fission dynamics cycle as observed in Metazoa was established. Expand
Reductive Evolution of the Mitochondrial Processing Peptidases of the Unicellular Parasites Trichomonas vaginalis and Giardia intestinalis
TLDR
The computational and experimental/functional analyses reveal that the divergent processing peptidases of Giardia mitosomes and Trichomonas hydrogenosomes evolved from the same ancestral heterodimeric α/βMPP metallopeptidase as did the classic mitochondrial enzyme. Expand
The Minimal Proteome in the Reduced Mitochondrion of the Parasitic Protist Giardia intestinalis
TLDR
The small proteome of the Giardia mitosome reflects the reduction in mitochondrial metabolism, which is limited to the FeS cluster assembly pathway, and a simplicity in the protein import pathway required for organelle biogenesis. Expand
Mitosomes in Parasitic Protists
TLDR
Finding the latter function in mitosomes supports the notion that FeS cluster assembly is the only essential function of mitochondria necessary for the maturation of cellular FeS proteins. Expand
Secretory protein trafficking in Giardia intestinalis
TLDR
Encysting Giardia provide a unique system for the study of unconventional, Golgi‐independent protein trafficking mechanisms in the broader context of eukaryotic endomembrane organization and evolution. Expand
A Single Tim Translocase in the Mitosomes of Giardia intestinalis Illustrates Convergence of Protein Import Machines in Anaerobic Eukaryotes
TLDR
Analysis of TIM complexes across eukaryote diversity suggests that a “single Tim” translocase is a convergent adaptation of mitosomes in anaerobic protists, with Tim22 and Tim17 (but not Tim23), providing the protein backbone. Expand
Novel mitochondrion‐related organelles in the anaerobic amoeba Mastigamoeba balamuthi
TLDR
The data indicate that the loss of classical aerobic mitochondrial functions and acquisition of anaerobic enzymes and Fe‐S cluster assembly proteins occurred in a free‐living member of the eukaryote super‐kingdom Amoebozoa. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 77 REFERENCES
A mitochondrial-like chaperonin 60 gene in Giardia lamblia: evidence that diplomonads once harbored an endosymbiont related to the progenitor of mitochondria.
  • A. Roger, S. Svärd, +4 authors M. Sogin
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1998
TLDR
The most parsimonious interpretation of these data is that the cpn60 gene was transferred from the endosymbiotic ancestors of mitochondria to the nucleus early in eukaryotic evolution, before the divergence of the diplomonads and trichomonads from other extant eUKaryotic lineages. Expand
The mitosome, a novel organelle related to mitochondria in the amitochondrial parasite Entamoeba histolytica
TLDR
The observed functional conservation between mitochondrial import in trypanosomes and mitosome import in Entamoeba is strong evidence that the E. histolytica organelle housing chaperonin CPN60 represents a mitochondrial remnant. Expand
Mitochondrial type iron-sulfur cluster assembly in the amitochondriate eukaryotes Trichomonas vaginalis and Giardia intestinalis, as indicated by the phylogeny of IscS.
TLDR
The phylogeny indicates the presence of a common mechanism for FeS cluster formation in mitochondriates as well as in amitochondriate eukaryotes, and supports a common origin of Trichomonas hydrogenosomes and mitochondria, aswell as secondary loss of mitochondrion/hydrogenosome-like organelles in Giardia. Expand
A mitochondrial remnant in the microsporidian Trachipleistophora hominis
TLDR
It is shown that a highly specific antibody raised against a Trachipleistophora hominis Hsp70 protein detects the presence of numerous tiny organelles with double membranes in this human microsporidial parasite, providing further evidence of the reluctance of eukaryotes to lose the mitochondrial organelle, even when its canonical function of aerobic respiration has been apparently lost. Expand
Biology of Giardia lamblia
  • R. D. Adam
  • Medicine, Biology
  • Clinical Microbiology Reviews
  • 2001
TLDR
The Giardia genome project promises to greatly increase the understanding of this interesting and enigmatic organism. Expand
Biogenesis of iron-sulphur clusters in amitochondriate and apicomplexan protists.
  • F. Seeber
  • Biology, Medicine
  • International journal for parasitology
  • 2002
TLDR
The current view of [Fe-S] cluster biogenesis in eukaryotes is summarized and its implications for amitochondriate protists and for the plastid-like organelle of apicomplexan parasites are discussed. Expand
Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi
TLDR
The DNA sequences of the 11 chromosomes of the ∼2.9-megabase (Mb) genome of Encephalitozoon cuniculi are reported and it is hypothesize that microsporidia have retained a mitochondrion-derived organelle. Expand
Giardia intestinalis, a eukaryote without hydrogenosomes, produces hydrogen.
TLDR
This first report of hydrogen production in a eukaryote with no recognizable hydrogenosomes raises further questions about the early branching status of G. intestinalis; the physiological characterization of its hydrogenase, and its recently elucidated gene sequence, will aid further phylogenetic investigations. Expand
Incorporation of iron into Tritrichomonas foetus cell compartments reveals ferredoxin as a major iron-binding protein in hydrogenosomes.
The intracellular transport of iron and its incorporation into organelles are poorly understood processes in eukaryotes and virtually unknown in parasitic protists. The transport of iron is ofExpand
Giardia lamblia expresses a proteobacterial-like DnaK homolog.
TLDR
A novel gene encoding molecular chaperone HSP70 in the amitochondriate parasite Giardia lamblia is identified and a specific relationship with the alpha proteobacterial and mitochondrial sequences was not strongly supported by phylogenetic analyses of this data set, in contrast to similar analyses of cpn60. Expand
...
1
2
3
4
5
...