Mitochondrial membrane potential measurement in rat cerebellar neurons by flow cytometry.

@article{Sureda1997MitochondrialMP,
  title={Mitochondrial membrane potential measurement in rat cerebellar neurons by flow cytometry.},
  author={Francesc X Sureda and E Escubedo and Cec{\'i}lia Gabriel and Jaume Comas and Jordi Camarasa and Antoni Camins},
  journal={Cytometry},
  year={1997},
  volume={28 1},
  pages={74-80}
}
Mitochondrial membrane potential (MMP) in dissociated rat cerebellar neurons was measured using rhodamine 123 (Rh 123) as fluorescent dye, and flow cytometry. Dye distribution was studied by confocal scanning microscopy. Propidium iodide (PI)-marked cells (dead cells) were not stained by Rh 123, while the green fluorescence of living cells was restricted to mitochondria. Incubation of cells with different ionophores resulted in a maximal inhibition of Rh 123 fluorescence of 27.0 +/- 5.9… CONTINUE READING
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