Mitochondrial iron not bound in heme and iron-sulfur centers. Estimation, compartmentation and redox state.

@article{Tangers1980MitochondrialIN,
  title={Mitochondrial iron not bound in heme and iron-sulfur centers. Estimation, compartmentation and redox state.},
  author={A Tanger{\aa}s and Torgeir Flatmark and Dan Baeckstr{\"o}m and Anders Ehrenberg},
  journal={Biochimica et biophysica acta},
  year={1980},
  volume={589 2},
  pages={162-75}
}
A method is described for the assay of total mitochondrial non-heme iron and a fraction which does not belong to the iron-sulfur proteins (FeS centers) of the outer and inner membrane. The assay of the latter fraction, which is termed 'non-heme non-FeS iron', is based on the formation of a chelate of Fe(II) with bathophenanthroline sulfonate in osmotically swollen mitochondria under conditions where the FeS centers are quite stable as determined by EPR spectroscopy at 20.4 K, 93 K and 123 K… CONTINUE READING

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