Mitochondrial inhibition in rat retinal cell cultures as a model of metabolic compromise: mechanisms of injury and neuroprotection.

@article{Wood2012MitochondrialII,
  title={Mitochondrial inhibition in rat retinal cell cultures as a model of metabolic compromise: mechanisms of injury and neuroprotection.},
  author={John P. M. Wood and Teresa Mammone and Glyn Chidlow and T. J. Greenwell and Robert J. Casson},
  journal={Investigative ophthalmology & visual science},
  year={2012},
  volume={53 8},
  pages={4897-909}
}
PURPOSE Our study aimed to establish a model of energetic and metabolic dysfunction to cultured retinal cells by chemically inhibiting the mitochondrial electron transport chain with sodium azide (NaN(3)), and subsequently investigating toxic mechanisms and potential neuroprotective strategies. Methods. Mixed rat retinal cultures comprising neurons and glia were treated with a range of NaN(3) concentrations for up to 24 hours and toxicity levels were determined by immunologic METHODS Detailed… CONTINUE READING