Mitochondrial carbonic anhydrase.

  title={Mitochondrial carbonic anhydrase.},
  author={Susanna J Dodgson and Robert E. Forster and Bayard T. Storey and Leena Mela},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={77 9},
We have assayed carbonic anhydrase activity (carbonate dehydratase, carbonate hydro-lyase, EC and bicarbonate permeability in suspensions of broken and intact guinea pig mitochondria by monitoring the disappearance of C16O18O. We found significant activity in preparations from liver and skeletal muscle, but not in preparations from heart muscle, brain, and kidney. Intact mitochondria containing carbonic anhydrase produce a two-phase acceleration of the disappearance of the labeled CO2… CONTINUE READING