Mitochondrial and nuclear DNA variability in the living fossil Triops cancriformis (Bosc, 1801) (Crustacea, Branchiopoda, Notostraca)

  title={Mitochondrial and nuclear DNA variability in the living fossil Triops cancriformis (Bosc, 1801) (Crustacea, Branchiopoda, Notostraca)},
  author={Barbara Mantovani and Michele Cesari and Andrea Luchetti and Franca Sabelli Scanabissi},
The living fossil Triops cancriformis comprises bisexual (either gonochoric or hermaphroditic) and unisexual populations. Genetic surveys have recently revealed a general trend of low differentiation of 12S and 16S mitochondrial genes. We, therefore, surveyed further mitochondrial (COI gene and control region) and nuclear markers (dinucleotide microsatellites) to assess the genetic variability and to establish any relationship with the different reproductive modes found in European populations… 

Genetic variability and reproductive modalities in European populations of Triops cancriformis (Crustacea, Branchiopoda, Notostraca)

Wright's F‐statistics, AMOVA, and ISOLDE analyses showed that populations are well structured and isolated, again as a result of different reproductive modalities, but also of geographic distances and low migration flow.

Population genetic structure and phylogenetic origin of Triops longicaudatus (Branchiopoda: Notostraca) on the Korean Peninsula

This work is the first comprehensive population genetic and phylogenetic approach for studying T. longicaudatus from East Asia and unambiguously corroborate that the Korean populations are subdivided into three distinct genetic lineages.

Sex ratio, reproductive mode and genetic diversity in Triops cancriformis

Results of marker segregation in eggs produced in isolation from non-gonochoric populations indicate that meiosis occurs and are consistent with two reproductive modes: selfing hermaphroditism and a type of ameiotic parthenogenesis.

R2 dynamics in Triops cancriformis (Bosc, 1801) (Crustacea, Branchiopoda, Notostraca): turnover rate and 28S concerted evolution

Results suggest that transposition-mediated deletion mechanisms, possibly involving genomic turnover processes acting on rDNAs, can dramatically decrease the copy number or even delete R2 from the ribosomal locus.

Evolutionary relationships within Triops (Branchiopoda: Notostraca) using complete mitochondrial genomes

Genetic differentiation among the genomes of Triops in New Mexico support elevation to species status andVariation in mitochondrial genes were identified that will aid future identification of cryptic lineages of tadpole shrimp.

Mitochondrial genome diversity and evolution in Branchiopoda (Crustacea)

Branchiopod mitogenomes appear rather well-conserved, although gene rearrangements have occurred, and the higher substitution rate of Anostraca and Onychocaudata is explained with the inferred GC substitution bias that occurred during their evolution.

Draft genomes and genomic divergence of two Lepidurus tadpole shrimp species (Crustacea, Branchiopoda, Notostraca)

The analysis demonstrates that, in these organisms, it is possible to obtain high‐quality draft genomes from single individuals with a relatively low sequencing effort, which makes Lepidurus and Notostraca interesting models for genomic studies at taxonomic, ecological and evolutionary levels.

Discriminating between the Effects of Founding Events and Reproductive Mode on the Genetic Structure of Triops Populations (Branchiopoda: Notostraca)

The high genetic differentiation between populations is likely due to founder effects and results suggest both species are composed of selfing hermaphrodites with some level of outcrossing; the presence of males in T. newberryi does not appreciably reduce inbreeding.

Morphologic and Genetic Variation in Triops (Branchiopoda: Notostraca) from Ephemeral Waters of the Northern Chihuahuan Desert of North America

The results support the presence of three morphologically distinct forms of tadpole shrimp, possibly three species in the genus Triops, which may be sufficiently distinct in morphology, mitochondrial DNA, and reproductive life history to warrant elevation to species level.

Toward a Global Phylogeny of the “Living Fossil" Crustacean Order of the Notostraca

A molecular phylogeny of the Notostraca was constructed using model based phylogenetic methods and it was concluded that, although some present day tadpole shrimp species closely resemble fossil specimens as old as 250 mya, no molecular support was found for an ancient (pre) Mesozoic radiation.



Chromosomes in sexual populations of Notostracan and Con-chostracan taxa (Crustacea, Branchiopoda)

The karyotype of the main Italian taxa, the Notostracan living fossils Triops cancriformis and Lepidurus apus lubbocki and E. ticinensis, shows a diploid number of 12 chromosomes, which is in line with previous results on other Italian populations, but contrasts with observations on European samples.

Populationen Von Triops Cancriformis (Bosc) (Notostraca) in Deutschland Nördlich 50°n sInd Nicht Klonal Und Höchstens Fakultativ Hermaphroditisch

It is suggested that Triops cancriformis populations in Germany north of 50°N are not clonal and at best facultatively hermaphroditic, and the structure of the eleventh pair of legs turned out to be the only suitable one for discriminating the gender of (fixed) Triops material.

Molecular characterization of the tadpole shrimp Triops (Branchiopoda: Notostraca) from the Baja California Peninsula, México: New insights on species diversity and phylogeny of the genus

Molecular and phylogenetic analyses indicate that the nominal Triops species T. longicaudatus is a mixture of several species such that, of the seven Triops American populations studied, six phylogenetic species can be identified and two morphologically and reproductively highly divergent forms can be grouped into a single monophyletic clade.

Phylogeography, phylogeny and hybridization in trichechid sirenians: implications for manatee conservation

The three living species of manatees, West Indian (Trichechus manatus), Amazonian (Trichechus inunguis) and West African (Trichechus senegalensis), are distributed across the shallow tropical and

Microsatellite variation in Daphnia pulex from both sides of the Baltic Sea

A study of variation at five microsatellite loci in a total of 34 populations from small ponds and rockpools on both sides of the Baltic Sea at 60–61°N, finding no conclusive evidence for obligative parthenogenesis.

Sister species within the Triops cancriformis lineage ( Crustacea , Notostraca )

This investigation is the first to demonstrate the presence of T. c.

Characterization of dinucleotide microsatellite loci in the living fossil tadpole shrimp Triops cancriformis (Crustacea Branchiopoda Notostrace)

Five dinucleotide markers were obtained from a microsatellite genomic library of the tadpole shrimp Triops cancriformis and heterozygosity levels were indicative of selfing in the studied populations whose real sexuality will be further tested using these markers.

Application of mitochondrial control region in population genetic studies of the shrimp Penaeus

Results indicate that the mitochondrial control region in shrimps provides more informative sites and reveals more haplotypes, making it most useful for evaluating genetic variations within and between populations of Penaeus species.


The morphological data support the full functionality of males in T. cancriformis, however, further work on gametogenesis, distribution of sexes, and genetic analysis of breeding experiments are needed to reach a fuller understanding of the role of male in middle and northern European populations of this species.

The mitochondrial nad2 gene as a novel marker locus for phylogenetic analysis of early land plants: a comparative analysis in mosses.

Both genes independently question the monophyly of the orders Dicranales and Neckerales and reject the inclusion of the genera Schistostega, Timmia, and Encalypta among Eubryales.