Mitochondrial and lysosomal protective agents ameliorate cytotoxicity and oxidative stress induced by cyclophosphamide and methotrexate in human blood lymphocytes

@article{Salimi2019MitochondrialAL,
  title={Mitochondrial and lysosomal protective agents ameliorate cytotoxicity and oxidative stress induced by cyclophosphamide and methotrexate in human blood lymphocytes},
  author={Ahmad Salimi and Roxanna Pirhadi and Zhaleh Jamali and Maral Ramazani and BS Yousefsani and Jalal Pourahmad},
  journal={Human \& Experimental Toxicology},
  year={2019},
  volume={38},
  pages={1266 - 1274}
}
Cyclophosphamide (CYP) and methotrexate (MTX) have been evaluated for their ability to induce toxicity in human peripheral blood lymphocytes (PBLs) and the protective role of mitochondrial and lysosomal stabilizing agents. The potential toxicity effects of CYP and MTX were measured in vitro by cellular parameters assays such as cellular viability, reactive oxygen species (ROS) formation, mitochondrial membrane permeability transition (mitochondrial membrane potential (MMP)) collapse, lysosomal… 
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42 References

Methotrexate induced mitochondrial injury and cytochrome c release in rat liver hepatocytes

It is suggested that MTX-induced cytotoxicity caused by ROS formation and GSH oxidation leads to oxidative stress and mitochondrial injury in rat hepatocytes.

Lutein Protects against Methotrexate-Induced and Reactive Oxygen Species-Mediated Apoptotic Cell Injury of IEC-6 Cells

The MTX-induced apoptosis of IEC-6 cells was shown to be repressed by the pre-treatment of lutein, which may represent a promising adjunct to conventional chemotherapy for preventing intestinal damages.

Toxicity of methyl tertiary-butyl ether on human blood lymphocytes

It was concluded that MTBE was capable of inducing oxidative stress and damage to mitochondria and lysosomes in human lymphocytes at concentrations ranging from 5 to 40 μg/L, which may be present in human blood as a result of environmental exposure.

Probucol Attenuates Cyclophosphamide-induced Oxidative Apoptosis, p53 and Bax Signal Expression in Rat Cardiac Tissues

  • Y. Asiri
  • Biology, Chemistry
    Oxidative medicine and cellular longevity
  • 2010
Probucol prevents the development of CP-induced cardiotoxicity by a mechanism related, at least in part, to its ability to increase mRNA expression of antioxidant genes and to decrease apoptosis in cardiac tissues with the consequent improvement in mitochondrial oxidative phosphorylation and energy production.

Protective effects of boron on cyclophosphamide induced lipid peroxidation and genotoxicity in rats.

Evaluation of cyclophosphamide-induced genotoxicity and cytotoxicity in cultured human lymphocytes

CP has genotoxic and cytotoxic effect on cultured human lymphocytes as compared to normal, and the frequency of micronuclei in binucleated cells showed a dose-dependent increase.

Protective effect of N-acetylcysteine on cyclophosphamide-induced cardiotoxicity in rats.

Age associated oxidative damage in lymphocytes

The results indicated that, balance of oxidant and antioxidant systems in lymphocytes shifts in favor of accelerated oxidative damage during aging, Thus oxidative stress in lymphocyte may particular interest in aging and may play important role in immunosenescence.

Cyclophosphamide-induced generation of reactive oxygen species. Comparison with morphological changes in type II alveolar epithelial cells and lung capillaries.

Mitochondrial Permeability Transition and Cell Death: The Role of Cyclophilin D

Direct and indirect mechanisms underlying CyP-D interaction with a target protein of the mPTP complex are discussed, which will help to further develop new pharmacological agents targeting mitochondria-mediated cell death.