Mitochondrial DNA sequences from a 7000-year old brain.

@article{Pbo1988MitochondrialDS,
  title={Mitochondrial DNA sequences from a 7000-year old brain.},
  author={Svante P{\"a}{\"a}bo and John A. Gifford and A. C. C. Wilson},
  journal={Nucleic acids research},
  year={1988},
  volume={16 20},
  pages={
          9775-87
        }
}
Pieces of mitochondrial DNA from a 7000-year-old human brain were amplified by the polymerase chain reaction and sequenced. Albumin and high concentrations of polymerase were required to overcome a factor in the brain extract that inhibits amplification. For this and other sources of ancient DNA, we find an extreme inverse dependence of the amplification efficiency on the length of the sequence to be amplified. This property of ancient DNA distinguishes it from modern DNA and thus provides a… Expand
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References

SHOWING 1-10 OF 17 REFERENCES
Molecular Cloning: A Laboratory Manual
TLDR
The content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories. Expand
The Colonisation of the Pacific: A Genetic Trail (Eds
  • Nature
  • 1987
Proc. Natl. Acad. Sci. USA
  • Proc. Natl. Acad. Sci. USA
  • 1988
J. Mol. Evol. Nature
  • J. Mol. Evol. Nature
  • 1987
J. World Prehist
  • J. World Prehist
  • 1987
Nucl. Acids Res
  • Nucl. Acids Res
  • 1987
Proc. Natl. Acad. Sci. USA
  • Proc. Natl. Acad. Sci. USA
  • 1987
Cold Spring Harbor Sympos
  • Quantit. Biol
  • 1986
Cold Spring Harbor Sympos
  • Quantit . Biol .
  • 1986
Human Genet
  • Human Genet
  • 1986
...
1
2
...