Mitochondrial DNA Analysis of the Japanese Wolf (Canis Lupus Hodophilax Temminck, 1839) and Comparison with Representative Wolf and Domestic Dog Haplotypes

  title={Mitochondrial DNA Analysis of the Japanese Wolf (Canis Lupus Hodophilax Temminck, 1839) and Comparison with Representative Wolf and Domestic Dog Haplotypes},
  author={Naotaka Ishiguro and Yasuo Inoshima and Nobuo Shigehara},
  booktitle={Zoological science},
Mitochondrial DNA (mtDNA) D-loop control region sequences ranging in length from 583 to 598 bp were determined for eight Japanese wolf specimens (Canis lupus hodophilax Temminck, 1839) collected from several sites and compared with 105 haplotypes from the domestic dog (C. lupus familiaris) and continental grey wolf (C. lupus lupus). Also, a 197-bp mtDNA sequence was amplified from archaeological wolf specimens and two continental wolf specimens (C. lupus chanco) as reference sequences for… 

Japanese Wolves are Genetically Divided into Two Groups Based on an 8-Nucleotide Insertion/Deletion within the mtDNA Control Region

The mitochondrial DNA (mtDNA) control region of four ancient Canis specimens was examined, and each specimen was genetically identified as Japanese wolf, and two unique nucleotide substitutions were observed in each sample.

Analysis of the Mitochondrial Genomes of Japanese Wolf Specimens in the Siebold Collection, Leiden

The taxonomic status of extinct Japanese or Honshu wolves has been disputed since the name hodophilax was first proposed by Temminck in 1839 and a mitochondrial genome analysis suggested that Japanese wolves could be categorized into two distinct clusters.

Biodiversity lost: The phylogenetic relationships of a complete mitochondrial DNA genome sequenced from the extinct wolf population of Sicily

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The Japanese wolf is most closely related to modern dogs and its ancestral genome has been widely inherited by dogs throughout East Eurasia

Genomic analyses indicate that the Japanese wolf was a unique subspecies of the gray wolf that was genetically distinct from both modern and ancientgray wolves, lacking gene flow with other gray wolves, and shows significant genetic affinities with East Eurasian dogs.

Origins of domestic dog in Southern East Asia is supported by analysis of Y-chromosome DNA

Y-chromosome and mtDNA data give strikingly similar pictures of dog phylogeography, most importantly that roughly 50% of the gene pools are shared universally but only ASY has nearly the full range of genetic diversity, such that the gene pool in all other regions may derive from ASY.

Osteological and Genetic Analysis of the Extinct Ezo Wolf (Canis Lupus Hattai) from Hokkaido Island, Japan

The morphological and genetic characters indicate that the ancestor of the Ezo wolf was genetically related to that of the grey wolf in Canada.

The Sicilian wolf: Genetic identity of a recently extinct insular population

All the wolf haplotypes detected in this study belonged to the mitochondrial haplogroup that includes haplotype detected in all the known European Pleistocene wolves and in several modern southern European populations.

DNA barcoding of three species (Canis aureus, Canis lupus and Vulpes vulpes) of Canidae

The validity of COI barcodes for detecting genetic divergence and to reveal whether or not there is a genetic variation at this marker within canids was tested.

Geographical Origin of the Domestic Dog

Recent genetic studies based on mtDNA suggest a single origin in Southeast Asia from numerous wolves less than 16 000 years ago as well as later hybridisation events in East Asia, the Middle East, Scandinavia and possibly North America.

The Sicilian Wolf: Genetic Identity of a Recently Extinct Insular Population.

To gain a better understanding of the genetic identity of the Sicilian wolf, techniques for the study of ancient DNA were used to analyze the mitochondrial (mt) variability of six specimens stored in Italian museums and found four of the samples shared the same haplotype.



Variations in Mitochondrial DNA of Dogs Isolated from Archaeological Sites in Japan and Neighbouring Islands

From results, it could not discern which modern Japanese dog breed closely resembles ancient dogs using phylogenetic analysis, but the CL1 cluster was likely distributed in the Japanese archipelago from the Jomon Period.

Extensive interbreeding occurred among multiple matriarchal ancestors during the domestication of dogs: evidence from inter- and intraspecies polymorphisms in the D-loop region of mitochondrial DNA between dogs and wolves.

The results suggested that the extant breeds of domestic dogs have maintained a large degree of mtDNA polymorphisms introduced from their ancestral wolf populations, and that extensive interbreedings had occurred among multiple matriarchal origins.

Multiple and ancient origins of the domestic dog.

Sequences from both dogs and wolves showed considerable diversity and supported the hypothesis that wolves were the ancestors of dogs, suggesting that dogs originated more than 100,000 years before the present.

Intra- and interbreed genetic variations of mitochondrial DNA major non-coding regions in Japanese native dog breeds (Canis familiaris).

Phylogenetic analysis showed that Japanese native dog breeds could not be clearly delimited as distinct breeds, and interbreed nucleotide differences between Japanese dog breeds were almost the same as the intrabreed nucleotide diversities.

Lineage classification of canine inheritable disorders using mitochondrial DNA haplotypes.

Estimating the maternal effects of dog breeds using mitochondrial DNA(mtDNA) haplotypes in the dogs with several clinical disorders revealed that each dog breed genetically comprises one or a few mtDNA haplotypes, but no disorder closely associated with mt DNA haplotypes was detected.

Ancient DNA Evidence for Old World Origin of New World Dogs

Mitochondrial DNA sequences isolated from ancient dog remains from Latin America and Alaska showed that native American dogs originated from multiple Old World lineages of dogs that accompanied late

Mammalian remains of the earliest Jomon period at the rockshelter site of Tochibara, Nagano., Japan

It was estimated that the size difference of the molars between the Jomon Period and the living specimens was the result of the climatic change that occured during this period.

Osteometrical and CT examination of the Japanese wolf skull.

The skulls of Japanese wolf (Canis hodophilax) were osteometrically examined and compared with those of Akita-Inu. The skull total length was not statistically different between two species. However,

MRI examination of the masticatory muscles in the gray wolf (Canis lupus), with special reference to the M. temporalis.

In the gray wolf, it is suggested that the M. temporalis may not be well-developed in terms of size of arising area, but in the thickness of running bundles, which is in accordance with the enlargement of the frontal bone and the increase in brain size.

The University Museum, The University of Tokyo