Mitochondrial Complex I superoxide production is attenuated by uncoupling.

  title={Mitochondrial Complex I superoxide production is attenuated by uncoupling.},
  author={Andrea Dlaskov{\'a} and Lydie Plecit{\'a} Hlavat{\'a} and Jan Je{\vz}ek and Petr Jezek},
  journal={The international journal of biochemistry & cell biology},
  volume={40 10},
Complex I, i.e. proton-pumping NADH:quinone oxidoreductase, is an essential component of the mitochondrial respiratory chain but produces superoxide as a side-reaction. However, conditions for maximum superoxide production or its attenuation are not well understood. Unlike for Complex III, it has not been clear whether a Complex I-derived superoxide generation at forward electron transport is sensitive to membrane potential or protonmotive force. In order to investigate this, we used Amplex Red… CONTINUE READING