Mitochondrial ATP synthesis in permeabilized cells: assessment of the ATP/O values in situ.

@article{Ouhabi1998MitochondrialAS,
  title={Mitochondrial ATP synthesis in permeabilized cells: assessment of the ATP/O values in situ.},
  author={R Ouhabi and M Boue-Grabot and J. P. Mazat},
  journal={Analytical biochemistry},
  year={1998},
  volume={263 2},
  pages={169-75}
}
We have developed a method to assess the actual mitochondrial ATP synthesis in cells (fibroblasts and Ehrlich ascites tumor cells). This method relies on gentle permeabilization of the cells, inhibition of nonmitochondrial ATP synthesis systems (glycolysis and adenylate kinase), and the inhibition of all ATPase activities (ATP hydrolysis) by decreasing the cytosolic magnesium concentration with EDTA. We have simultaneously measured the rate of respiration, and the ATP/O values obtained with… CONTINUE READING