Mismatch repair genes identified using genetic screens in Blm-deficient embryonic stem cells

@article{Guo2004MismatchRG,
  title={Mismatch repair genes identified using genetic screens in Blm-deficient embryonic stem cells},
  author={Ge Guo and Wei Wang and Allan Bradley},
  journal={Nature},
  year={2004},
  volume={429},
  pages={891-895}
}
Phenotype-driven recessive genetic screens in diploid organisms require a strategy to render the mutation homozygous. Although homozygous mutant mice can be generated by breeding, a reliable method to make homozygous mutations in cultured cells has not been available, limiting recessive screens in culture. Cultured embryonic stem (ES) cells provide access to all of the genes required to elaborate the fundamental components and physiological systems of a mammalian cell. Here we have exploited… CONTINUE READING
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