Miscibility phase diagrams of giant vesicles containing sphingomyelin.

@article{Veatch2005MiscibilityPD,
  title={Miscibility phase diagrams of giant vesicles containing sphingomyelin.},
  author={Sarah L. Veatch and Sarah L. Keller},
  journal={Physical review letters},
  year={2005},
  volume={94 14},
  pages={
          148101
        }
}
Saturated sphingomyelin (SM) lipids are implicated in lipid rafts in cell plasma membranes. Here we use fluorescence microscopy to observe coexisting liquid domains in vesicles containing SM, an unsaturated phosphatidylcholine lipid (either DOPC or POPC), and cholesterol. We note similar phase behavior in a model membrane mixture without SM (DOPC/DPPC/Chol), but find no micron-scale liquid domains in membranes of POPC/PSM/Chol. We delineate the onset of solid phases below the miscibility… 

Figures from this paper

Phase diagrams of lipid mixtures relevant to the study of membrane rafts.

Complex Phase Behavior of GUVs Containing Different Sphingomyelins.

Phase behavior and domain size in sphingomyelin-containing lipid bilayers.

Formation of ceramide/sphingomyelin gel domains in the presence of an unsaturated phospholipid: a quantitative multiprobe approach.

The complete ternary phase diagram is established, which helps to predict PCer-rich gel domain formation and explains its enhancement through PSM/PCer interactions.

Seeing spots: complex phase behavior in simple membranes.

Disrupting membrane raft domains by alkylphospholipids.

Cholesterol-induced fluid membrane domains: a compendium of lipid-raft ternary phase diagrams.

  • D. Marsh
  • Chemistry
    Biochimica et biophysica acta
  • 2009
...

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