Mirasol PRT treatment of donor white blood cells prevents the development of xenogeneic graft‐versus‐host disease in Rag2–/–γc–/– double knockout mice

@article{Fast2006MirasolPT,
  title={Mirasol PRT treatment of donor white blood cells prevents the development of xenogeneic graft‐versus‐host disease in Rag2–/–$\gamma$c–/– double knockout mice},
  author={Loren D Fast and Gilbert Dileone and Gene A. Cardarelli and Junzhi Li and Raymond P. Goodrich},
  journal={Transfusion},
  year={2006},
  volume={46}
}
BACKGROUND: Mirasol PRT (Navigant Biotechnologies) treatment utilizes exposure to light in the presence of riboflavin to introduce irreparable lesions to nucleic acids thereby inhibiting pathogen and WBC replication. The ability of Mirasol PRT–treated mononuclear cells (MNCs) to generate xenogeneic graft‐versus‐host disease (GVHD) responses was used to model transfusion‐associated GVHD (TAGVHD). 
Developments in the prevention of transfusion‐associated graft‐versus‐host disease
  • L. Fast
  • Biology, Medicine
  • British journal of haematology
  • 2012
TLDR
These methods have been found to be as effective as γ‐irradiation in preventing T lymphocyte proliferation and TA‐GVHD responses and additional benefits of pathogen reduction protocols potentially include inhibition of antigen presentation, cytokine production and activation of lymphocytes. Expand
Preventing transfusion-associated graft-versus-host disease: state of the art
TLDR
Comparison of pathogen reduction protocols with γ-irradiation have found that both protocols are equally effective in preventing T lymphocyte proliferation and GVHD responses when testing in both in vitro and in vivo models. Expand
Cesium cessation? An advantage of pathogen reduction treatments
TLDR
This editorial focuses on the principal regulatory, technical, and clinical challenges associated with using a free-standing CsCl source irradiator to treat blood components and presents some of the evidence supporting pathogen reduction treatment (PRT) as an alternative means of preventing TA-GVHD. Expand
Treatment of whole blood with riboflavin plus ultraviolet light, an alternative to gamma irradiation in the prevention of transfusion‐associated graft‐versus‐host disease?
TLDR
Exposure of blood products to gamma irradiation is currently the standard of care in the prevention of transfusion‐associated graft‐ associated graft‐versus‐host disease (TA‐GVHD). Expand
Histological assessment of the sclerotic graft-versus-host response in the humanized RAG2-/-γc-/- mouse model.
  • M. Hogenes, S. van Dorp, +5 authors R. D. de Weger
  • Biology, Medicine
  • Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation
  • 2012
TLDR
The combination of immunohistochemical cell characterization and mRNA expression analysis of both human (donor)- and murine (host)- derived cytokines reveals an interaction between host tissues and donor-derived cells in an entangled cytokine profile, in which both donor- and host-derived cytokines contribute to the formation of fibrosis. Expand
Transfusion‐associated graft‐versus‐host disease
Transfusion‐associated graft‐versus‐host disease (TA‐GvHD) is a rare complication of transfusion of cellular blood components producing a graft‐versus‐host clinical picture with concomitant boneExpand
Reduced MHC alloimmunization and partial tolerance protection with pathogen reduction of whole blood
TLDR
It is demonstrated that pathogen reduction of platelet‐rich plasma (PRP) with riboflavin and ultraviolet light (UV+R) can prevent alloimmunization in mice, and a similar pathogen‐reduction treatment is currently under development for the treatment of whole blood using ribofavin and a higher dose of UV light. Expand
Inactivation of human white blood cells in platelet products after pathogen reduction technology treatment in comparison to gamma irradiation
TLDR
The aim of this study was to compare the effect of PRT treatment to gamma irradiation and find out if PRT processes are well suited for WBC inactivation. Expand
White blood cell inactivation after treatment with riboflavin and ultraviolet light
TLDR
Data from in vitro studies and this clinical trial suggest that PRT treatment may be as effective as gamma irradiation in preventing TA‐GVHD and more effective than leukoreduction in preventing alloimmunization. Expand
Pathogen reduction and HLA alloimmunization: more questions than answers
TLDR
The authors tested for HLA Class I and Class II antibodies before and serially after transfusion and found that receipt of Mirasol-treated platelets was associated with a significantly increased rate of alloimmunization to anti-HLA Class I antigens using both the low and high assay cutoff points in an intention-to-treat analysis. Expand
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