Minor groove binder-conjugated DNA probes for quantitative DNA detection by hybridization-triggered fluorescence.

@article{Afonina2002MinorGB,
  title={Minor groove binder-conjugated DNA probes for quantitative DNA detection by hybridization-triggered fluorescence.},
  author={Irina Afonina and Michael W. Reed and E Lusby and I. G. Shishkina and Yevgeniy S Belousov},
  journal={BioTechniques},
  year={2002},
  volume={32 4},
  pages={
          940-4, 946-9
        }
}
Here we describe the properties of a novel class of oligonucleotide probes capable of sensitive hybridization-triggered fluorescence. These fluorogenic probes, known commercially as MGB Eclipse probes, are characterized by having a conjugated minor groove binder (MGB) ligand at the 5'-end and a fluorophore at the 3'-end. Additionally, they have an efficient quencher moiety at the 5'-end that is useful with a wide variety of fluorescent dyes. Fluorescence of the single-stranded MGB Eclipse probe… CONTINUE READING
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