Minimum information necessary for quantitative real-time PCR experiments.

  title={Minimum information necessary for quantitative real-time PCR experiments.},
  author={Gemma L. Johnson and Afif M. Abdel Nour and Tania Nolan and Jim F. Huggett and Stephen Bustin},
  journal={Methods in molecular biology},
The MIQE (minimum information for the publication of quantitative real-time PCR) guidelines were published in 2009 with the twin aims of providing a blueprint for good real-time quantitative polymerase chain reaction (qPCR) assay design and encouraging the comprehensive reporting of qPCR protocols. It had become increasingly clear that variable pre-assay conditions, poor assay design, and incorrect data analysis were leading to the routine publication of data that were often inconsistent… CONTINUE READING
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