Minimally invasive quantification of lymph flow in mice and rats by imaging depot clearance of near-infrared albumin.

@article{Karlsen2012MinimallyIQ,
  title={Minimally invasive quantification of lymph flow in mice and rats by imaging depot clearance of near-infrared albumin.},
  author={Tine Veronica Karlsen and Emmet Mccormack and Maja Muji{\'c} and Olav Tenstad and Helge Wiig},
  journal={American journal of physiology. Heart and circulatory physiology},
  year={2012},
  volume={302 2},
  pages={
          H391-401
        }
}
There is a lack of available methods to noninvasively quantify lymphatic function in small experimental animals, a necessity for studies on lymphatic system pathophysiology. We present a new method to quantify lymph flow in mice and rats, based on optically monitoring the depot clearance of near-infrared fluorescently labeled albumin and subsequent calculation of removal rate constants (k). BSA was conjugated with Alexa680 NHS ester and remained stable in protein-rich solutions without free dye… CONTINUE READING
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