Minimally invasive determination of mRNA concentration in single living bacteria

@inproceedings{Guet2008MinimallyID,
  title={Minimally invasive determination of mRNA concentration in single living bacteria},
  author={Călin C Guet and Luke Bruneaux and Taejin L. Min and Dan Siegal-Gaskins and Israel Figueroa and Thierry Emonet and Philippe Cluzel},
  booktitle={Nucleic acids research},
  year={2008}
}
Fluorescence correlation spectroscopy (FCS) has permitted the characterization of high concentrations of noncoding RNAs in a single living bacterium. Here, we extend the use of FCS to low concentrations of coding RNAs in single living cells. We genetically fuse a red fluorescent protein (RFP) gene and two binding sites for an RNA-binding protein, whose translated product is the RFP protein alone. Using this construct, we determine in single cells both the absolute [mRNA] concentration and the… CONTINUE READING