Minimally-invasive Technique for Injection into Rat Optic Nerve.

  title={Minimally-invasive Technique for Injection into Rat Optic Nerve.},
  author={Kateryna Raykova and Melina V. Jones and Hwa Huang and Paul F Hoffman and Michael Levy},
  journal={Journal of visualized experiments : JoVE},
The rat optic nerve is a useful model for stem cell regeneration research. Direct injection into the rat optic nerve allows delivery into the central nervous system in a minimally-invasive surgery without bone removal. This technique describes an approach to visualization and direct injection of the optic nerve following minor fascial dissection from the orbital ridge, using a conjunctival traction suture to gently pull the eye down and out. Representative examples of an injected optic nerve… CONTINUE READING
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