Minimal sequence and structural requirements of a subgenomic RNA promoter for turnip crinkle virus.
@article{Wang1999MinimalSA,
title={Minimal sequence and structural requirements of a subgenomic RNA promoter for turnip crinkle virus.},
author={J Wang and Clifford D. Carpenter and Anne E. Simon},
journal={Virology},
year={1999},
volume={253 2},
pages={
327-36
}
}Infection of plants or protoplasts with turnip crinkle virus (TCV) results in the synthesis of the genomic RNA and two subgenomic (sg) RNAs of 1.7 kb and 1.45 kb, respectively. Both of the sgRNA promoters were characterized previously and their secondary structures predicted by computer analysis [J. Wang and A. E. Simon (1997). Virology 232, 174-186]. Secondary structure-sensitive chemical and enzymatic probes have now been used to determine the structure of the promoter directing synthesis of…
47 Citations
A conserved hairpin structure in Alfamovirus and Bromovirus subgenomic promoters is required for efficient RNA synthesis in vitro.
- BiologyRNA
- 2000
Analysis of the subgenomic promoter (sgp) in minus-strand RNA 3 showed that a sequence of 37 nt upstream of the RNA 4 start site was sufficient for full sgp activity in an in vitro assay with the purified viral RNA-dependent RNA-polymerase (RdRp).
Analyses of Subgenomic Promoters of Hibiscus Chlorotic Ringspot Virus and Demonstration of 5′ Untranslated Region and 3′-Terminal Sequences Functioning as Subgenomic Promoters
- BiologyJournal of Virology
- 2006
This is the first demonstration that both the 5′ untranslated region (UTR) and part of the 3′ UTR can be duplicated and function as sgRNA promoters within a single viral genome.
A cis-replication element functions in both orientations to enhance replication of Turnip crinkle virus.
- BiologyVirology
- 2006
A Sequence-Independent, Unstructured Internal Ribosome Entry Site Is Responsible for Internal Expression of the Coat Protein of Turnip Crinkle Virus
- BiologyJournal of Virology
- 2017
It is demonstrated that a region of viral RNA devoid of extensive secondary structure has IRES activity and produces low levels of viral coat protein in vitro and in vivo.
Identification and Functional Analysis of the Turnip Yellow Mosaic Tymovirus Subgenomic Promoter
- BiologyJournal of Virology
- 2000
It is reported that the subgenomic promoter sequence of TYMV is located on a 494-nucleotide fragment, containing previously identified highly conserved sequence elements, shown here to be essential for promoter function.
Mapping of the Tobacco mosaic virus movement protein and coat protein subgenomic RNA promoters in vivo.
- BiologyVirology
- 2000
The Tobacco mosaic virus movement protein (MP) and coat protein (CP) are expressed from 3'-coterminal subgenomic RNAs (sgRNAs) and deletion analysis suggested that the upper part of this stem-loop, located upstream of the transcription start site, was essential for transcription and that the lower part of the stem had an enhancing role.
Evidence supporting a premature termination mechanism for subgenomic RNA transcription in Pelargonium line pattern virus: identification of a critical long-range RNA-RNA interaction and functional variants through mutagenesis.
- BiologyThe Journal of general virology
- 2016
Insight into the mechanism that governs PLPV sg mRNA transcription is aimed at getting insights into the mechanisms underlying premature termination, which seems to occur in the plus-strand, likely acts in cis, and specifically mediates the synthesis of sg RNA-sized minus-Strand.
Structural Analysis and Whole Genome Mapping of a New Type of Plant Virus Subviral RNA: Umbravirus-Like Associated RNAs
- BiologyViruses
- 2021
The biological and structural characterization of umbravirus-like associated RNAs (ulaRNAs), a new category of coat-protein dependent subviral RNA replicons that infect plants, lays down a road map for future investigations into these novel virus-like RNAs.
Requirement of a 5'-proximal linear sequence on minus strands for plus-strand synthesis of a satellite RNA associated with turnip crinkle virus.
- BiologyVirology
- 2000
In vivo genetic selection revealed a strict requirement for 10 of the 14 nucleotides of the element, indicating that the primary sequence is essential for RNA accumulation.
References
SHOWING 1-10 OF 42 REFERENCES
Analysis of the two subgenomic RNA promoters for turnip crinkle virus in vivo and in vitro.
- BiologyVirology
- 1997
Computer generated secondary structures for the two sgRNA promoters revealed an extensive hairpin just upstream from the transcription start site, which indicates higher sequence conservation for the 1.45-kb sg RNA promoter compared with the 2.7-kb ssgRNA promoter, despite the latter's location within the RNA-dependent RNA polymerase open reading frame.
RNA promoters located on (-)-strands of a subviral RNA associated with turnip crinkle virus.
- BiologyRNA
- 1997
This result suggests that multiple consecutive C residues are important for transcription initiation and that natural 3'-end sequences, when located at 3' termini, help the RdRp to initiate at the 3' end of the molecule.
Mutational analysis of the core and modulator sequences of the BMV RNA3 subgenomic promoter.
- BiologyNucleic acids research
- 1988
Homologies to sequences surrounding the initiation sites of subgenomic RNAs from several plant RNA viruses, and from alphaviruses, have been detected.
Structure and assembly of turnip crinkle virus. IV. Analysis of the coat protein gene and implications of the subunit primary structure.
- BiologyJournal of molecular biology
- 1987
Sequence-specific recognition of a subgenomic RNA promoter by a viral RNA polymerase.
- BiologyProceedings of the National Academy of Sciences of the United States of America
- 1997
It is shown that the RdRp of the plant-infected BMV is capable of accurately, albeit inefficiently, initiating RNA synthesis from the subgenomic promoter of the animal-infecting Semliki Forest virus.
Analysis of sequences and predicted structures required for viral satellite RNA accumulation by in vivo genetic selection.
- BiologyNucleic acids research
- 1998
The utility of the in vivo selection approach is demonstrated by showing that both sequence and structure contribute to a more active 3'-end region for accumulation of sat-RNA C.
Analysis in vivo of turnip crinkle virus satellite RNA C variants with mutations in the 3'-terminal minus-strand promoter.
- BiologyVirology
- 1997
Turnip crinkle virus and its associated RNA, sat-RNA C, share similar, but not identical hairpins near their 3' ends and terminate with CCUGCCC-OH, which forms a single-stranded tail. With an in…
Characterization of sequences controlling the synthesis of alfalfa mosaic virus subgenomic RNA in vivo.
- BiologyVirology
- 1995
Engineering of point mutations and small deletions in RNA 3 around the transcription start site for RNA 4 synthesis revealed elements important for sg promoter activity with similarity to sequences conserved in sg promoters of alpha-like viruses.
Characterization and engineering of sequences controlling in vivo synthesis of brome mosaic virus subgenomic RNA
- BiologyJournal of virology
- 1988
Subgenomic RNA production at normal levels required sequences extending to at least -74 but not beyond -95, and loss of an (rA)18 tract immediately upstream of the -20 to +16 "core promoter" particularly inhibited subgenomicRNA synthesis.



