Minimal machinery of RNA polymerase holoenzyme sufficient for promoter melting.

@article{Young2004MinimalMO,
  title={Minimal machinery of RNA polymerase holoenzyme sufficient for promoter melting.},
  author={Brian A. Young and Tanja Mo Gruber and Carol A. Gross},
  journal={Science},
  year={2004},
  volume={303 5662},
  pages={1382-4}
}
We determined the minimal portion of Escherichia coli RNA polymerase (RNAP) holoenzyme able to accomplish promoter melting, the crucial step in transcription initiation that provides RNAP access to the template strand. Upon duplex DNA binding, the N terminus of the beta' subunit (amino acids 1 to 314) and amino acids 94 to 507 of the sigma subunit, together comprising less than one-fifth of RNAP holoenzyme, were able to melt an extended -10 promoter in a reaction remarkably similar to that of… CONTINUE READING

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