Minimal Ras-binding domain of Raf1 can be used as an activation-specific probe for Ras

@article{Rooij1997MinimalRD,
  title={Minimal Ras-binding domain of Raf1 can be used as an activation-specific probe for Ras},
  author={Johan de Rooij and J. L. Bos},
  journal={Oncogene},
  year={1997},
  volume={14},
  pages={623-625}
}
Ras is a small GTPase that cycles between an inactive GDP-bound and an active GTP-bound form. A large variety of ligands that stimulate cell surface receptors induce the activation of Ras. Thus far, this activation could only be measured by the increase of GTP bound to Ras, which was precipitated from radio-labelled cell extract. We have used the minimal Ras-binding domain (RBD) of Raf1 (aa 51-131) to identify in vivo activated Ras. This novel method is based on the observation that RBD binds… CONTINUE READING
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