Minimal Handling and Super-Binary Vectors Facilitate Efficient, Agrobacterium-Mediated, Transformation of Sugarcane (Saccharum spp. hybrid)

Abstract

Agrobacterium-mediated transformation (AMT) of sugarcane has been limited by low transformation efficiency, high variability between experiments and genotype specificity. We tested combinations of parameters that have been useful in other recalcitrant plant systems, aiming to develop an efficient and reproducible method. Applied to elite sugarcane cultivar… (More)
DOI: 10.1007/s12042-012-9101-1

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