Minimal Escherichia coli cell for the most efficient production of ethanol from hexoses and pentoses.

Abstract

To obtain an efficient ethanologenic Escherichia coli strain, we reduced the functional space of the central metabolic network, with eight gene knockout mutations, from over 15,000 pathway possibilities to 6 pathway options that support cell function. The remaining pathways, identified by elementary mode analysis, consist of four pathways with non-growth… (More)
DOI: 10.1128/AEM.02708-07

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