Minimal, encapsulated proteomic-sample processing applied to copy-number estimation in eukaryotic cells

@article{Kulak2014MinimalEP,
  title={Minimal, encapsulated proteomic-sample processing applied to copy-number estimation in eukaryotic cells},
  author={Nils Alexander Kulak and Garwin Pichler and Igor Paron and Nagarjuna Nagaraj and Matthias Mann},
  journal={Nature Methods},
  year={2014},
  volume={11},
  pages={319-324}
}
Mass spectrometry (MS)-based proteomics typically employs multistep sample-preparation workflows that are subject to sample contamination and loss. We report an in-StageTip method for performing sample processing, from cell lysis through elution of purified peptides, in a single, enclosed volume. This robust and scalable method largely eliminates contamination or loss. Peptides can be eluted in several fractions or in one step for single-run proteome analysis. In one day, we obtained the… CONTINUE READING
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