Minibody: A novel engineered anti-carcinoembryonic antigen antibody fragment (single-chain Fv-CH3) which exhibits rapid, high-level targeting of xenografts.
@article{Hu1996MinibodyAN, title={Minibody: A novel engineered anti-carcinoembryonic antigen antibody fragment (single-chain Fv-CH3) which exhibits rapid, high-level targeting of xenografts.}, author={S. Hu and L. Shively and A. Raubitschek and M. Sherman and L. Williams and J. Wong and J. Shively and A. Wu}, journal={Cancer research}, year={1996}, volume={56 13}, pages={ 3055-61 } }
A novel engineered antibody fragment (VL-VH-CH3, or "minibody") with bivalent binding to carcinoembryonic antigen (CEA) was produced by genetic fusion of a T84.66 (anti-CEA) single-chain antibody (scFv) to the human IgG1 CH3 domain. Two designs for the connecting peptide were evaluated. In the T84.66/212 LD minibody, a two-amino acid linker (generated by fusion of restriction sites) was used to join VH and CH3. In the T84.66/212 Flex minibody, the human IgG1 hinge plus an additional 10 residues… Expand
Figures, Tables, and Topics from this paper
323 Citations
Pilot Trial Evaluating an 123I-Labeled 80-Kilodalton Engineered Anticarcinoembryonic Antigen Antibody Fragment (cT84.66 Minibody) in Patients with Colorectal Cancer
- Medicine
- Clinical Cancer Research
- 2004
- 76
- PDF
Construction of divalent anti-keratin 8 single-chain antibodies (sc(Fv)(2)), expression in Pichia pastoris and their reactivity with multicellular tumor spheroids.
- Chemistry, Medicine
- Journal of immunological methods
- 2011
- 10
Improved binding of a bivalent single-chain immunotoxin results in increased efficacy for in vivo T-cell depletion.
- Biology, Medicine
- Protein engineering
- 2001
- 51
- Highly Influenced
- PDF
Construction and purification of a covalently linked divalent tandem single-chain Fv antibody against placental alkaline phosphatase.
- Chemistry, Medicine
- Hybridoma
- 2006
- 8
Construction and characterization of a novel recombinant single-chain variable fragment antibody against Western equine encephalitis virus.
- Biology, Medicine
- Hybridoma
- 2000
- 21
The crystal structure of an anti-CEA scFv diabody assembled from T84.66 scFvs in V(L)-to-V(H) orientation: implications for diabody flexibility.
- Chemistry, Medicine
- Journal of molecular biology
- 2003
- 60
Expression and purification of recombinant immunotoxin--a fusion protein stabilizes a single-chain Fv (scFv) in denaturing condition.
- Biology, Medicine
- Protein expression and purification
- 2003
- 6
Molecular modification of a recombinant anti-CD3epsilon-directed immunotoxin by inducing terminal cysteine bridging enhances anti-GVHD efficacy and reduces organ toxicity in a lethal murine model.
- Biology, Medicine
- Blood
- 2000
- 13
Preparation of humanized ovarian carcinoma anti-idiotypic minibody.
- Biology, Medicine
- Hybridoma and hybridomics
- 2003
- 7
References
SHOWING 1-10 OF 46 REFERENCES
Tumor localization of anti-CEA single-chain Fvs: improved targeting by non-covalent dimers.
- Chemistry, Medicine
- Immunotechnology : an international journal of immunological engineering
- 1996
- 211
Recombinant single-chain Fv fragments carrying C-terminal cysteine residues: production of bivalent and biotinylated miniantibodies.
- Chemistry, Medicine
- Molecular immunology
- 1994
- 91
Biological properties of chimeric domain-deleted anticarcinoma immunoglobulins.
- Biology, Medicine
- Cancer research
- 1995
- 58
- Highly Influential
- PDF
Comparative stabilities in vitro and in vivo of a recombinant mouse antibody FvCys fragment and a bisFvCys conjugate.
- Biology, Medicine
- Journal of immunology
- 1992
- 74
Construction, binding properties, metabolism, and tumor targeting of a single-chain Fv derived from the pancarcinoma monoclonal antibody CC49.
- Biology, Medicine
- Cancer research
- 1991
- 348
- Highly Influential
- PDF
Spontaneous assembly of bivalent single chain antibody fragments in Escherichia coli.
- Biology, Medicine
- Molecular immunology
- 1994
- 96
Serum half-life and tumor localization of a chimeric antibody deleted of the CH2 domain and directed against the disialoganglioside GD2.
- Biology, Medicine
- Proceedings of the National Academy of Sciences of the United States of America
- 1990
- 81
- PDF
In vivo tumor targeting of a recombinant single-chain antigen-binding protein.
- Biology, Medicine
- Journal of the National Cancer Institute
- 1990
- 299
Rapid tumor penetration of a single-chain Fv and comparison with other immunoglobulin forms.
- Medicine
- Cancer research
- 1992
- 808
- PDF
Miniantibodies: use of amphipathic helices to produce functional, flexibly linked dimeric FV fragments with high avidity in Escherichia coli.
- Biology, Medicine
- Biochemistry
- 1992
- 301
- PDF