Millimolar concentrations of ascorbic acid in purified human mononuclear leukocytes. Depletion and reaccumulation.

@article{Bergsten1990MillimolarCO,
  title={Millimolar concentrations of ascorbic acid in purified human mononuclear leukocytes. Depletion and reaccumulation.},
  author={Peter Bergsten and Gabriel Amitai and John H. Kehrl and Kuldeep R. Dhariwal and Harvey G Klein and Mark A Levine},
  journal={The Journal of biological chemistry},
  year={1990},
  volume={265 5},
  pages={
          2584-7
        }
}
Ascorbic acid (vitamin C) was found in isolated human mononuclear leukocytes and their purified components in millimolar concentration. Intracellular ascorbic acid was depleted greater than 96% during cell culture and was rapidly reaccumulated after addition of physiologic concentrations of ascorbic acid to the extracellular medium. Purified cells maintained concentration gradients of ascorbic acid as large as 100-fold across the plasma membrane. The ability to vary intracellular ascorbic acid… Expand
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The preferential uptake of the dehydro-form of the vitamin may be useful for situations where this short-lived metabolite is formed by oxidation in the environment. Expand
Assessing the reductive capacity of cells by measuring the recycling of ascorbic and lipoic acids.
  • J. M. May
  • Chemistry, Medicine
  • Methods in molecular biology
  • 2010
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Methods have been developed to assess the ability of intact cells to recycle ascorbate and to generate intracellular reducing equivalents required to maintain the redox status of the cells. Expand
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Application of vitamin C to T cells in vitro did not recapitulate previously reported in vivo responses to vitamin C, suggesting that in vivo, vitamin C modulates T cells indirectly through other components of the microenvironment. Expand
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The various pathways of regeneration of ascorbate and their relative contributions to the avoidance of vitamin loss in plasma or cell culture medium are discussed. Expand
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