Millimolar concentrations of ascorbic acid in purified human mononuclear leukocytes. Depletion and reaccumulation.

@article{Bergsten1990MillimolarCO,
  title={Millimolar concentrations of ascorbic acid in purified human mononuclear leukocytes. Depletion and reaccumulation.},
  author={Peter Bergsten and Gabriel Amitai and John H. Kehrl and Kuldeep R. Dhariwal and Harvey G Klein and Mark A Levine},
  journal={The Journal of biological chemistry},
  year={1990},
  volume={265 5},
  pages={
          2584-7
        }
}
Ascorbic acid (vitamin C) was found in isolated human mononuclear leukocytes and their purified components in millimolar concentration. Intracellular ascorbic acid was depleted greater than 96% during cell culture and was rapidly reaccumulated after addition of physiologic concentrations of ascorbic acid to the extracellular medium. Purified cells maintained concentration gradients of ascorbic acid as large as 100-fold across the plasma membrane. The ability to vary intracellular ascorbic acid… Expand
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