Milking the genome for profit

  title={Milking the genome for profit},
  author={Alan W. Dove},
  journal={Nature Biotechnology},
  • A. Dove
  • Published 1 October 2000
  • Biology
  • Nature Biotechnology
Despite recent accomplishments, significant challenges remain before cloning can become a routine part of transgenic animal production. 
Advances in the identification of Quantitative Trait Loci and genes that influence agronomically important traits and the use of this novel information in socalled marker assisted selection (MAS) are discussed.
Transgenic Animals: Principles, Methods and Applications
This chapter focuses on the basics of technology, methods, application and future prospects of transgenic animals in medicine, agriculture and industry. Transgenic technologies and the ability to
Transgenic Goats in the World Pharmaceutical Industry of the 21st Century
The role of fundamental science in the development of this field of the pharmaceutical industry is discussed, and an anticoagulant antithrombin III developed by Genzyme Transgenic Corporation will appear on the pharmaceutical market in the nearest future.
Animal Biopharming in New Zealand Drivers, Scenarios and Practical Implications
Constructive Conversations/Korero Whakaaetanga (Phase 2): Biopharming, Risk Assessment and Regulation. Research Report no.12.
The generation method of transgenic avian is established using retroviral vectors for gene delivery and the germ-line transmission efficiency of G0 quails mated with non-transgenic quails was more than 80%.
Fish can be First – Advances in Fish Transgenesis for Commercial Applications
  • H. Zbikowska
  • Biology, Environmental Science
    Transgenic Research
  • 2004
‘All-fish’ growth hormone gene constructs and antifreeze protein genes have been successfully introduced into the fish genome resulting in a significant acceleration of growth rate and an increase in cold and freeze tolerance.
The Transgenic Rabbit as Model for Human Diseases and as a Source of Biologically Active Recombinant Proteins
Until recently, transgenic rabbits were produced exclusively by pronuclear microinjection which results in additive random insertional transgenesis; however, progress in somatic cell cloning based on
The development and application of genome editing technology in ruminants: a review
It is concluded that the production of gene-edited ruminants is feasible and through improvements in genome editing technology it is possible to help feed the world.
Effect of Transgenesis on the Morphology and Function of the Rabbit Mammary Gland
Genome manipulation and microinjection of WAP gene structure – hFVIII in transgenic rabbit embryos did not significantly affect mammary gland tissues morphology of lactating rabbits, and does not have significant expression in other organs.
The Making of “Transgenic Spermatozoa”1
The different aspects of sperm-mediated transgenesis are presented and the advantage of being able to create transgenic progeny simply by mating with wild-type females, which avoids the possibility of interference or damage as a result of assisted fertilization or the manipulation of embryos.


Pig cloning by microinjection of fetal fibroblast nuclei.
To clone pigs from differentiated cells, microinjected the nuclei of porcine (Sus scrofa) fetal fibroblasts into enucleated oocytes, and development was induced by electroactivation, which produced an apparently normal female piglet.
Production of transgenic rabbits, sheep and pigs by microinjection
Integration of the fusion gene in all three species and expression of the gene in transgenic rabbits and pigs are reported here.
Transgene vectors go retro
In this approach, pronuclei (vesicles containing the maternal and paternal genes in a fertilized egg), or nuclei of two-cell embryos, are microinjected with DNA, resulting in delivery of several hundred transgene copies, which guarantees that all animals born are transgenic.
Cloned pigs produced by nuclear transfer from adult somatic cells
The successful production of cloned piglets from a cultured adult somatic cell population using a new nuclear transfer procedure is reported and the methodology used for embryo reconstruction in each of these species is essentially similar.
Transformation of mouse emybro by pronuclear injection
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Generation of rabbits by nuclear transfer
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Generation of transgenic cattle by sperm vectors
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