Mih1/Cdc25 is negatively regulated by Pkc1 in Saccharomyces cerevisiae.

  title={Mih1/Cdc25 is negatively regulated by Pkc1 in Saccharomyces cerevisiae.},
  author={Kouitiro Yano and Yukifumi Uesono and Satoshi Yoshida and Akihiko Kikuchi and Jun Kashiwazaki and Issei Mabuchi and Yoshiko Kikuchi},
  journal={Genes to cells : devoted to molecular & cellular mechanisms},
  volume={18 6},
Mitotic cyclin-dependent kinase (CDK) is activated by Cdc25 phosphatase through dephosphorylation at the Wee1-mediated phosphorylation site. In Saccharomyces cerevisiae, regulation of Mih1 (Cdc25 homologue) remains unclear because inactivation/degradation of Swe1 (Wee1 homologue) is the main trigger for G2/M transition. By deleting all mitotic cyclins except Clb2, a strain was created where Mih1 became essential for mitotic entry at high temperatures. Using this novel assay, the essential… CONTINUE READING
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