Migration of breast epithelial cells on Laminin-5: differential role of integrins in normal and transformed cell types

@article{Plopper1998MigrationOB,
  title={Migration of breast epithelial cells on Laminin-5: differential role of integrins in normal and transformed cell types},
  author={George E. Plopper and Susan Z. Domanico and Vincenzo Cirulli and William B. Kiosses and Vito Quaranta},
  journal={Breast Cancer Research and Treatment},
  year={1998},
  volume={51},
  pages={57-69}
}
We examined the role of Laminin-5 (Ln-5) an extracellular matrix component of breast gland basement membrane, in supporting migration of normal (HUMEC), immortalized (MCF-10A), and malignant breast epithelial cells that exhibit different degrees of metastatic potential (MDA-MB-435>MDA-MB-231>MCF-7). HUMEC, MCF-10A, and MCF-7 cells all adhered to purified Ln-5 through the α3β1 integrin receptor in adhesion assays. However, HUMEC and MCF-10A cells remained statically adherent, while MCF-7 cells… CONTINUE READING