Migration of Escherichia coli dnaB protein on the template DNA strand as a mechanism in initiating DNA replication.

@article{McMacken1977MigrationOE,
  title={Migration of Escherichia coli dnaB protein on the template DNA strand as a mechanism in initiating DNA replication.},
  author={Roger McMacken and Kazumitsu Ueda and Arthur Kornberg},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1977},
  volume={74 10},
  pages={4190-4}
}
The first step in conversion of varphiX174 singlestranded DNA to the duplex replicative form in vitro is the synthesis of a nucleoprotein intermediate [Weiner, J. H., McMacken, R. & Kornberg, A. (1976) Proc. Natl. Acad. Sci. USA 73, 752-756]. We now demonstrate that dnaB protein (approximately one molecule per DNA circle) is an essential component of the intermediate and retains its ATPase activity. Synthesis of RNA primers, dependent on dnaG protein (primase), occurred only on DNA that had… CONTINUE READING

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