Midgut proteins released by microapocrine secretion in Spodoptera frugiperda.

@article{Silva2013MidgutPR,
  title={Midgut proteins released by microapocrine secretion in Spodoptera frugiperda.},
  author={Walciane da Silva and Christiane Cardoso and Alberto F C Ribeiro and Walter R Terra and Cl{\'e}lia Ferreira},
  journal={Journal of insect physiology},
  year={2013},
  volume={59 1},
  pages={
          70-80
        }
}
Microapocrine vesicles bud from the lepidopteran midgut microvilli as double membrane vesicles. To identify the proteins secreted by this process, antibodies raised against isolated microapocrine vesicles from Spodoptera frugiperda were used for screening a midgut cDNA expression library. Positive clones were sequenced, assembled and N blasted against S. frugiperda sequences obtained by pyrosequencing midgut mRNA. This procedure led to the extension of microapocrine sequences that were… CONTINUE READING
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