Middle cerebral artery occlusion in the rat by intraluminal suture. Neurological and pathological evaluation of an improved model.

@article{Belayev1996MiddleCA,
  title={Middle cerebral artery occlusion in the rat by intraluminal suture. Neurological and pathological evaluation of an improved model.},
  author={Ludmila Belayev and Ofelia Furones Alonso and Raul Busto and W. Zhao and Myron D. Ginsberg},
  journal={Stroke},
  year={1996},
  volume={27 9},
  pages={
          1616-22; discussion 1623
        }
}
BACKGROUND AND PURPOSE The purpose of the present study was to evaluate a modified method of intraluminal suture occlusion of the middle cerebral artery (MCA) on the volume of brain infarction and on neurobehavioral function in rats subjected to a temporary focal ischemic insult. METHODS Male Sprague-Dawley rats were anesthetized with halothane and subjected to 60 minutes or 2 hours of temporary MCA occlusion (MCAo) by an intraluminal thread. In one group of rats, the suture was coated with… 

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Does poly-L-lysine coating of the middle cerebral artery occlusion suture improve infarct consistency in a murine model?

Transient middle cerebral artery occlusion in rats as an experimental model of brain ischemia.

The experimental model induced significant ischemic cerebral injury in both groups and resulted in significant neurological deficit score for the 90 and 120 min groups.
...

References

SHOWING 1-10 OF 42 REFERENCES

Correlation between cerebral blood flow and histologic changes in a new rat model of middle cerebral artery occlusion.

A new focal ischemia model consisting of unilateral middle cerebral artery occlusion with a silicone rubber cylinder attached to a nylon surgical thread inserted through the internal carotid artery in rats that should be useful in various investigations of the influence of reperfusion on focal ischemic brain injury.

Reversible middle cerebral artery occlusion without craniectomy in rats.

Seven of eight rats that underwent permanent occlusion of the MCA had resolving moderately severe neurologic deficits and unilateral infarcts averaging 37.6 +/- 5.5% of the coronal sectional area at 72 hours after the onset of Occlusion.

Photothrombotic Occlusion of Rat Middle Cerebral Artery: Histopathological and Hemodynamic Sequelae of Acute Recanalization

Cortical neurons appear to tolerate 1 h of MCA occlusion in this model, and reperfusion following 1H of photochemically induced MCA Occlusion gives rise to selective injury to the striatum.

Middle Cerebral Artery Occlusion Without Craniectomy in Rats: Which Method Works Best?

The depth of ischemia is more profound, occlusion is achieved in a much higher proportion of cases, and the incidence of perforation of the intracranial internal carotid is much less frequent than by Longa's method.

Rat middle cerebral artery occlusion: evaluation of the model and development of a neurologic examination.

We have examined the incidence and size of infarction after occlusion of different portions of the rat middle cerebral artery (MCA) in order to define the reliability and predictability of this model

Recovery of Sensorimotor Function After Distal Middle Cerebral Artery Photothrombotic Occlusion in Rats

It is suggested that a battery of tests is necessary to fully characterize the pattern of behavioral deficits after focal cerebral ischemia, and location of infarct damage and associated degree of hemispheric atrophy were important variables in determining behavioral outcome.

Progression from ischemic injury to infarct following middle cerebral artery occlusion in the rat.

The gross pattern of the brain lesion and the maturation of neuronal changes typical of a brain infarct have a predictable progression and focal brain ischemia of up to 6-hour duration does not induce coagulation necrosis.

A New Model of Temporary Focal Neocortical Ischemia in the Rat

A new rat model of temporary focal ischemia that produces neocortical ischemies without the need for prolonged anesthesia is described, and experimental therapies aimed at lessening infarct size or restoring blood flow must be initiated within this critical time interval.

Focal Cerebral Ischaemia in the Rat: 1. Description of Technique and Early Neuropathological Consequences following Middle Cerebral Artery Occlusion

The ability to produce a consistent focal ischaemic lesion in the rodent brain provides a technical approach that is sufficiently reproducible to enable investigation of the pathophysiology of ischaemia using recently developed autoradiographic and neurochemical methods.

A reproducible model of middle cerebral infarcts, compatible with long-term survival, in aged rats.

It is concluded that reproducible MCA infarcts can be produced in aged rats by craniotomy and that these lesions may be compatible with long-term survival.