Microwave oven and boiling waterbath extraction of hepatotoxins from cyanobacterial cells.

@article{Metcalf2000MicrowaveOA,
  title={Microwave oven and boiling waterbath extraction of hepatotoxins from cyanobacterial cells.},
  author={J S Metcalf and Geoffrey A. Codd},
  journal={FEMS microbiology letters},
  year={2000},
  volume={184 2},
  pages={
          241-6
        }
}
Low-cost, straightforward methods for the extraction of microcystins and nodularins from cyanobacterial cells were developed using a microwave oven and boiling waterbath. The use of organic solvents, such as methanol, which can interfere with sensitive analytical procedures, e.g. immunoassays, can thus be avoided. Analysis by protein phosphatase inhibition assay and high performance liquid chromatography indicated that purified microcystin-LR was unaffected by the microwave oven and boiling… CONTINUE READING

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