Microwave or autoclave treatments destroy the infectivity of infectious bronchitis virus and avian pneumovirus but allow detection by reverse transcriptase-polymerase chain reaction.

@article{Elhafi2004MicrowaveOA,
  title={Microwave or autoclave treatments destroy the infectivity of infectious bronchitis virus and avian pneumovirus but allow detection by reverse transcriptase-polymerase chain reaction.},
  author={Giuma Elaref Elhafi and Clive J. Naylor and Carol E Savage and Richard Charles Jones},
  journal={Avian pathology : journal of the W.V.P.A},
  year={2004},
  volume={33 3},
  pages={
          303-6
        }
}
A method is described for enabling safe transit of denatured virus samples for polymerase chain reaction (PCR) identification without the risk of unwanted viable viruses. Cotton swabs dipped in avian infectious bronchitis virus (IBV) or avian pneumovirus (APV) were allowed to dry. Newcastle disease virus and avian influenza viruses were used as controls. Autoclaving and microwave treatment for as little as 20 sec destroyed the infectivity of all four viruses. However, both IBV and APV could be… CONTINUE READING

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