Microwave-mediated synthesis of labeled nucleotides with utility in the synthesis of DNA probes.

@article{Lefever2010MicrowavemediatedSO,
  title={Microwave-mediated synthesis of labeled nucleotides with utility in the synthesis of DNA probes.},
  author={Mark R Lefever and Jerome W. Kosmeder and Michael Farrell and Christopher Bieniarz},
  journal={Bioconjugate chemistry},
  year={2010},
  volume={21 10},
  pages={1773-8}
}
A novel method of linking haptens to deoxycytidine 5'-triphosphate via microwave-mediated bisulfate-catalyzed transamination with hydrazine has been developed. This method enables the tethering of small molecule haptens to dCTP via a discrete polyethylene glycol (PEG) spacer, yielding N(4)-aminodeoxycytidine 5'-triphosphate-dPEG-haptens. This synthetic approach employs microwave-catalyzed hydrazinolysis that enables the attachment of spacers via hydrazine linkages. The microwave-mediated… CONTINUE READING

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