Microwave fixation improves antigenicity of glutaraldehyde-sensitive antigens while preserving ultrastructural detail.

Microwave fixation for electron microscopy has been used primarily for post-embedding immunocytochemistry. The present study examined the ability of microwave fixation to preserve the antigenicity of glutaraldehyde-sensitive antigens for pre-embedding immunocytochemistry. Five monoclonal antibodies (MAbs) directed against cell surface components of rat mast… CONTINUE READING