Microwave-assisted deglycosylation for rapid and sensitive analysis of N-glycans via glycosylamine derivatization.

Abstract

A rapid and sensitive N-glycan profiling strategy for MALDI-MS incorporating the use of deglycosylation with microwave assistance and the co-derivatization of glycosylamine labeling with tris(2,4,6-trimethoxyphenyl)phosphonium acetic acid N-hydroxysuccinimide ester (TMPP-Ac-OSu) and methylamidation has been developed in this work. Notably, highly efficient… (More)
DOI: 10.1007/s00216-017-0346-2

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