Microwave-assisted acid hydrolysis of proteins combined with liquid chromatography MALDI MS/MS for protein identification.

Abstract

Simple and efficient digestion of proteins, particularly hydrophobic membrane proteins, is of significance for comprehensive proteome analysis using the bottom-up approach. We report a microwave-assisted acid hydrolysis (MAAH) method for rapid protein degradation for peptide mass mapping and tandem mass spectrometric analysis of peptides for protein… (More)

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