Microwave antigen retrieval of beta-amyloid precursor protein immunoreactivity.

@article{Sherriff1994MicrowaveAR,
  title={Microwave antigen retrieval of beta-amyloid precursor protein immunoreactivity.},
  author={F. E. Sherriff and L. R. Bridges and Paul Jackson},
  journal={Neuroreport},
  year={1994},
  volume={5 9},
  pages={1085-8}
}
Formalin fixation reduces beta-amyloid precursor protein (beta APP) immunoreactivity which restricts its study in archival tissue. Formic acid pretreatment has previously been used in an attempt to overcome this problem, but makes the sections very friable. In the present study, a microwave antigen retrieval method has been compared with formic acid pretreatment for retrieving beta APP immunoreactivity in formalin-fixed, paraffin-embedded human brain tissue. Microwave treatment resulted in… CONTINUE READING